Analysis of nutrient-dependent transcript variations in Neurospora crassa.
نویسندگان
چکیده
Nutrient-dependent variations in transcript levels of the filamentous fungus Neurospora crassa were studied on a microarray containing some 4700 cDNAs. Cells were grown in minimal and acetate medium. The isolated RNA was analyzed in comparison to the results obtained upon the hybridization of samples prepared from the RNA of cells grown in full medium. Altogether, 160 cDNA clones exhibited significant variations, falling into five distinct subgroups of very similar transcription profiles. This is indicative of the occurrence of a high degree of co-regulation of genes in N. crassa. Especially the regulation of the expression of proteins involved in metabolic pathways was found to be strongly regulated at the RNA level.
منابع مشابه
Biotransformation of Hydrocortisone by Neurospora crassa
The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...
متن کاملMOLECULAR ANALYSIS OF THE SULFUR REGULATORY CIRCUIT OF NEUROSPORA CRASSA
The sulfur regulatory circuit of the filamentous fungus, Neurospora crassa, consists of a set of unlinked structural genes which encode sulfur catabolic and two major regulatory genes which govern their expression. The cys-3 regulatory gene encode a transacting regulatory protein which activates the expression of cys-14 and ars, whereas the other regulatory genes Scon-l and Scon-2 appear to...
متن کاملGenetic and molecular characterization of a cryptochrome from the filamentous fungus Neurospora crassa.
In plants and animals, cryptochromes function as either photoreceptors or circadian clock components. We have examined the cryptochrome from the filamentous fungus Neurospora crassa and demonstrate that Neurospora cry encodes a DASH-type cryptochrome that appears capable of binding flavin adenine dinucleotide (FAD) and methenyltetrahydrofolate (MTHF). The cry transcript and CRY protein levels a...
متن کاملPhotoregulation of cot-1, a Kinase-Encoding Gene Involved in Hyphal Growth in Neurospora crassa.
Blue light plays a key role as an environmental signal in the regulation of growth and development of fungi and plants. Here we demonstrate that in Neurospora crassa hyphae branch more frequently in cultures grown in light. Previous studies had identified cot-1 as a gene that controls apical hyphal cell elongation. In the cot-1 mutant, cessation of elongation is accompanied by hyperbranching. H...
متن کاملIsolation of nit-4, the minor nitrogen regulatory gene which mediates nitrate induction in Neurospora crassa.
Expression of nitrate reductase in Neurospora crassa requires the positive action of nit-4, a pathway-specific regulatory gene, which mediates nitrate induction. We report the molecular cloning of the nit-4 gene and present results which suggest that the nit-4 gene is constitutively expressed to yield a low-abundance 2.2-kilobase transcript. These results indicate that the nit-2 major control g...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Fungal genetics and biology : FG & B
دوره 40 3 شماره
صفحات -
تاریخ انتشار 2003